Click image to see more details
Product Info Summary
| SKU: | RP1033 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Collagen III/COL3A1 Antibody
View all Collagen III alpha 1/COL3A1 Antibodies
SKU/Catalog Number
RP1033
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Collagen III/COL3A1 Antibody catalog # RP1033. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen III/COL3A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1033)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen III, different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1033 is reactive to COL3A1 in Human
Applications
RP1033 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
138.564kDa
Background of Collagen III alpha 1/COL3A1
COL3A1, also called EDS4A or Collagen alpha-1 (III), is a protein that in humans is encoded by the COL3A1 gene, it is mapped to 2q32.2. COL3A1 chain is a fibrillar-forming collagen comprising 3 alpha-1 (III) chains and is expressed in early embryos and throughout embryogenesis. In adult, COL3A1 is a major component of the extracellular matrix in a variety of internal organs and skin. COL3A1 is also a fibrous scleroprotein in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. It is involved in regulation of cortical development, and it Is the major ligand of GPR56 in the developing brain.COL3A1 binding to GPR56 can inhibit neuronal migration and activate the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-Collagen III Picoband antibody, RP1033-2.jpg
All lanes: Anti Collagen III(RP1033) at 0.5ug/ml
Lane 1: MCF-4 Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 139KD
Observed bind size: 139KD
Click image to see more details
Anti-Collagen III Picoband antibody, RP1033-1.jpg
All lanes: Anti Collagen III (RP1033) at 0.5ug/ml
WB: Recombinant Human Collagen III Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Protein Target Info & Infographic
Gene/Protein Information For COL3A1 (Source: Uniprot.org, NCBI)
Gene Name
COL3A1
Full Name
Collagen alpha-1(III) chain
Weight
138.564kDa
Superfamily
fibrillar collagen family
Alternative Names
alpha1 (III) collagen; COL3A1; Collagen 3; collagen alpha-1(III) chain; Collagen III alpha 1; collagen, fetal; collagen, type III, alpha 1; Collagen-3; EDS4A; Ehlers-Danlos syndrome type IV, autosomal dominant; FLJ34534 COL3A1 EDS4A, EDSVASC, PMGEDSV collagen type III alpha 1 chain collagen alpha-1(III) chain|Ehlers-Danlos syndrome type IV, autosomal dominant|alpha-1 type III collagen|alpha1 (III) collagen|collagen, fetal|collagen, type III, alpha 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COL3A1, check out the COL3A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COL3A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Collagen III/COL3A1 Antibody (RP1033)
Hello CJ!
RP1033 has been cited in 26 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xu L,Sun N,Li G,Liu L.LncRNA H19 promotes keloid formation through targeting the miR-769-5p/EIF3A pathway.Mol Cell Biochem.2021 Jan 3.doi:10.1007/s11010-020-04024-x.Epub ahead of print.PMID:33389493.
Species: Human
RP1033 usage in article: APP:WB, SAMPLE:KELOID FIBROBLASTS, DILUTION:1:1000
BMP-7 suppresses excessive scar formation by activating the BMP-7/Smad1/5/8 signaling pathway
Long-term intravenous administration of carboxylated single-walled carbon nanotubes induces persistent accumulation in the lungs and pulmonary fibrosis via the nuclear factor-kappa B pathway.
Role of transforming growth factor ?-1 in the pathogenesis of pelvic organ prolapse: A potential therapeutic target
Effects of rhBNP on myocardial fibrosis after myocardial infarction in rats
Continuing Treatment with Salvia miltiorrhiza Injection Attenuates Myocardial Fibrosis in Chronic Iron-Overloaded Mice
Mechanisms of Nerve Capping Technique in Prevention of Painful Neuroma Formation
Effects of pentoxifylline on the hepatic content of TGF-?1 and collagen in Schistosomiasis japonica mice with liver fibrosis
Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway
Astragalus membranaceus?ameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition?in vivo?and?in vitro
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Collagen III/COL3A1 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Collagen III/COL3A1 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
15 Customer Q&As for Anti-Collagen III/COL3A1 Antibody
Question
Would RP1033 anti-Collagen III/COL3A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
You can see on the product datasheet, RP1033 anti-Collagen III/COL3A1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-13
Question
Do you have a BSA free version of anti-Collagen III/COL3A1 antibody RP1033 available?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Collagen III/COL3A1 antibody RP1033 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-31
Question
Our lab want to know about using your anti-Collagen III/COL3A1 antibody for response to cytokine studies. Has this antibody been tested with western blotting on colo320 whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
Thanks for your inquiry. This RP1033 anti-Collagen III/COL3A1 antibody is validated on colo320 whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-18
Question
My question regarding product RP1033, anti-Collagen III/COL3A1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1033 anti-Collagen III/COL3A1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-11
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon carcinoma using anti-Collagen III/COL3A1 antibody RP1033. Let me know if you need anything else.
N. Edwards
Verified customer
Asked: 2019-08-06
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-06
Question
My question regards to test anti-Collagen III/COL3A1 antibody RP1033 on human colon carcinoma for research purposes, then I may be interested in using anti-Collagen III/COL3A1 antibody RP1033 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
The products we sell, including anti-Collagen III/COL3A1 antibody RP1033, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-07
Question
Will anti-Collagen III/COL3A1 antibody RP1033 work for WB with colon carcinoma?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
According to the expression profile of colon carcinoma, COL3A1 is highly expressed in colon carcinoma. So, it is likely that anti-Collagen III/COL3A1 antibody RP1033 will work for WB with colon carcinoma.
Boster Scientific Support
Answered: 2019-05-20
Question
Is a blocking peptide available for product anti-Collagen III/COL3A1 antibody (RP1033)?
E. Patel
Verified customer
Asked: 2019-01-08
Answer
We do provide the blocking peptide for product anti-Collagen III/COL3A1 antibody (RP1033). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-01-08
Question
See attached the WB image, lot number and protocol we used for colon carcinoma using anti-Collagen III/COL3A1 antibody RP1033. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-09-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-13
Question
I see that the anti-Collagen III/COL3A1 antibody RP1033 works with WB, what is the protocol used to produce the result images on the product page?
P. Wu
Verified customer
Asked: 2018-08-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-08-21
Question
We were well pleased with the WB result of your anti-Collagen III/COL3A1 antibody. However we have been able to see positive staining in uterine cervix secreted using this antibody. Is that expected? Could you tell me where is COL3A1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-02-01
Answer
According to literature, uterine cervix does express COL3A1. Generally COL3A1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have COL3A1 expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 7864881
Liver, Pubmed ID: 15489334, 24275569
Placenta, Pubmed ID: 3405773
Skin fibroblast, Pubmed ID: 2764886
Boster Scientific Support
Answered: 2018-02-01
Question
We are currently using anti-Collagen III/COL3A1 antibody RP1033 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-15
Answer
The anti-Collagen III/COL3A1 antibody (RP1033) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-15
Question
Is this RP1033 anti-Collagen III/COL3A1 antibody reactive to the isotypes of COL3A1?
Verified Customer
Verified customer
Asked: 2017-07-18
Answer
The immunogen of RP1033 anti-Collagen III/COL3A1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen III(1200-1221aa VGAAAIAGIGGEKAGGFAPYYG), different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-18
Question
I was wanting to use your anti-Collagen III/COL3A1 antibody for WB for human colon carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human colon carcinoma identification?
L. Williams
Verified customer
Asked: 2017-05-04
Answer
You can see on the product datasheet, RP1033 anti-Collagen III/COL3A1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human colon carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-05-04
Question
We have been able to see staining in human colon carcinoma. Are there any suggestions? Is anti-Collagen III/COL3A1 antibody supposed to stain colon carcinoma positively?
R. Miller
Verified customer
Asked: 2014-10-17
Answer
From what I have seen in literature colon carcinoma does express COL3A1. From what I have seen in Uniprot.org, COL3A1 is expressed in uterine cervix, skin fibroblast, liver, placenta, colon carcinoma, among other tissues. Regarding which tissues have COL3A1 expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 7864881
Liver, Pubmed ID: 15489334, 24275569
Placenta, Pubmed ID: 3405773
Skin fibroblast, Pubmed ID: 2764886
Boster Scientific Support
Answered: 2014-10-17
